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canine osteoblast differentiation medium  (Cell Applications Inc)
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Tri-lineage differentiation ability of induced pluripotent stem cell (iPSC)-derived mesenchymal stem cells (iMSCs) obtained using the neural crest cell (NCC) + PRIME protocol. iMSCs were obtained from canine embryonic fibroblast (CEF)-derived iPSCs (CEF-iPSCs), canine urine-derived cell (cUC)-derived iPSCs (cUC-iPSCs), and canine peripheral blood mononuclear cell (cPBMC)-derived iPSCs (cPBMC-iPSCs) using the NCC + PRIME protocol. The origin of PRIME-iMSCs is represented on the upper side of the pictures. (A) Von Kossa staining after <t>osteoblast</t> induction. Von Kossa staining identified hydroxyapatite crystals in the extracellular matrix of differentiated cells. Scale bar = 100 μm. qPCR for osteogenic markers, SPP1 and BGLAP , were shown below the staining. Black bars and white bars represent iMSCs before and after differentiation. Data are presented as relative quantification (RQ) for iMSCs before differentiation and as the mean ± standard deviation (n = 3). ∗p < 0.05. (B) Oil Red O staining after adipocyte differentiation. The cells formed Oil Red O-positive lipid vacuoles in the cytoplasm. Scale bar = 20 μm. qPCR for adipogenic marker, PLIN1 , was shown below the staining. Black bars and white bars represent iMSCs before and after differentiation. Data are presented as RQ for iMSCs before differentiation and as the mean ± standard deviation (n = 3). (C) Images of Alcian blue staining after chondrocyte induction. The iMSCs from all iPSC lines formed spheres and exhibited Alcian blue-positive proteoglycan production. Nuclei were stained red using nuclear fast red stain. Scale bar = 50 μm. qPCR for chondrogenic markers, ACAN and SOX9 , were shown below the picture. Black bars and white bars represent iMSCs before and after differentiation. Data are presented as RQ for iMSCs before differentiation and as the mean ± standard deviation (n = 3).
Canine Osteoblast Differentiation Medium, supplied by Cell Applications Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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osteoblast differentiation  (MedChemExpress)
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MedChemExpress osteoblast differentiation
Tri-lineage differentiation ability of induced pluripotent stem cell (iPSC)-derived mesenchymal stem cells (iMSCs) obtained using the neural crest cell (NCC) + PRIME protocol. iMSCs were obtained from canine embryonic fibroblast (CEF)-derived iPSCs (CEF-iPSCs), canine urine-derived cell (cUC)-derived iPSCs (cUC-iPSCs), and canine peripheral blood mononuclear cell (cPBMC)-derived iPSCs (cPBMC-iPSCs) using the NCC + PRIME protocol. The origin of PRIME-iMSCs is represented on the upper side of the pictures. (A) Von Kossa staining after <t>osteoblast</t> induction. Von Kossa staining identified hydroxyapatite crystals in the extracellular matrix of differentiated cells. Scale bar = 100 μm. qPCR for osteogenic markers, SPP1 and BGLAP , were shown below the staining. Black bars and white bars represent iMSCs before and after differentiation. Data are presented as relative quantification (RQ) for iMSCs before differentiation and as the mean ± standard deviation (n = 3). ∗p < 0.05. (B) Oil Red O staining after adipocyte differentiation. The cells formed Oil Red O-positive lipid vacuoles in the cytoplasm. Scale bar = 20 μm. qPCR for adipogenic marker, PLIN1 , was shown below the staining. Black bars and white bars represent iMSCs before and after differentiation. Data are presented as RQ for iMSCs before differentiation and as the mean ± standard deviation (n = 3). (C) Images of Alcian blue staining after chondrocyte induction. The iMSCs from all iPSC lines formed spheres and exhibited Alcian blue-positive proteoglycan production. Nuclei were stained red using nuclear fast red stain. Scale bar = 50 μm. qPCR for chondrogenic markers, ACAN and SOX9 , were shown below the picture. Black bars and white bars represent iMSCs before and after differentiation. Data are presented as RQ for iMSCs before differentiation and as the mean ± standard deviation (n = 3).
Osteoblast Differentiation, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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statistical analysis of staining for differentiated adipocyte and osteoblast  (S2 Statistical Solutions)
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S2 Statistical Solutions statistical analysis of staining for differentiated adipocyte and osteoblast
Tri-lineage differentiation ability of induced pluripotent stem cell (iPSC)-derived mesenchymal stem cells (iMSCs) obtained using the neural crest cell (NCC) + PRIME protocol. iMSCs were obtained from canine embryonic fibroblast (CEF)-derived iPSCs (CEF-iPSCs), canine urine-derived cell (cUC)-derived iPSCs (cUC-iPSCs), and canine peripheral blood mononuclear cell (cPBMC)-derived iPSCs (cPBMC-iPSCs) using the NCC + PRIME protocol. The origin of PRIME-iMSCs is represented on the upper side of the pictures. (A) Von Kossa staining after <t>osteoblast</t> induction. Von Kossa staining identified hydroxyapatite crystals in the extracellular matrix of differentiated cells. Scale bar = 100 μm. qPCR for osteogenic markers, SPP1 and BGLAP , were shown below the staining. Black bars and white bars represent iMSCs before and after differentiation. Data are presented as relative quantification (RQ) for iMSCs before differentiation and as the mean ± standard deviation (n = 3). ∗p < 0.05. (B) Oil Red O staining after adipocyte differentiation. The cells formed Oil Red O-positive lipid vacuoles in the cytoplasm. Scale bar = 20 μm. qPCR for adipogenic marker, PLIN1 , was shown below the staining. Black bars and white bars represent iMSCs before and after differentiation. Data are presented as RQ for iMSCs before differentiation and as the mean ± standard deviation (n = 3). (C) Images of Alcian blue staining after chondrocyte induction. The iMSCs from all iPSC lines formed spheres and exhibited Alcian blue-positive proteoglycan production. Nuclei were stained red using nuclear fast red stain. Scale bar = 50 μm. qPCR for chondrogenic markers, ACAN and SOX9 , were shown below the picture. Black bars and white bars represent iMSCs before and after differentiation. Data are presented as RQ for iMSCs before differentiation and as the mean ± standard deviation (n = 3).
Statistical Analysis Of Staining For Differentiated Adipocyte And Osteoblast, supplied by S2 Statistical Solutions, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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osteoblast differentiation medium  (Cell Applications Inc)
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Cell Applications Inc osteoblast differentiation medium
Tri-lineage differentiation ability of induced pluripotent stem cell (iPSC)-derived mesenchymal stem cells (iMSCs) obtained using the neural crest cell (NCC) + PRIME protocol. iMSCs were obtained from canine embryonic fibroblast (CEF)-derived iPSCs (CEF-iPSCs), canine urine-derived cell (cUC)-derived iPSCs (cUC-iPSCs), and canine peripheral blood mononuclear cell (cPBMC)-derived iPSCs (cPBMC-iPSCs) using the NCC + PRIME protocol. The origin of PRIME-iMSCs is represented on the upper side of the pictures. (A) Von Kossa staining after <t>osteoblast</t> induction. Von Kossa staining identified hydroxyapatite crystals in the extracellular matrix of differentiated cells. Scale bar = 100 μm. qPCR for osteogenic markers, SPP1 and BGLAP , were shown below the staining. Black bars and white bars represent iMSCs before and after differentiation. Data are presented as relative quantification (RQ) for iMSCs before differentiation and as the mean ± standard deviation (n = 3). ∗p < 0.05. (B) Oil Red O staining after adipocyte differentiation. The cells formed Oil Red O-positive lipid vacuoles in the cytoplasm. Scale bar = 20 μm. qPCR for adipogenic marker, PLIN1 , was shown below the staining. Black bars and white bars represent iMSCs before and after differentiation. Data are presented as RQ for iMSCs before differentiation and as the mean ± standard deviation (n = 3). (C) Images of Alcian blue staining after chondrocyte induction. The iMSCs from all iPSC lines formed spheres and exhibited Alcian blue-positive proteoglycan production. Nuclei were stained red using nuclear fast red stain. Scale bar = 50 μm. qPCR for chondrogenic markers, ACAN and SOX9 , were shown below the picture. Black bars and white bars represent iMSCs before and after differentiation. Data are presented as RQ for iMSCs before differentiation and as the mean ± standard deviation (n = 3).
Osteoblast Differentiation Medium, supplied by Cell Applications Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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osteoblast differentiation medium  (Lonza)
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Lonza osteoblast differentiation medium
Tri-lineage differentiation ability of induced pluripotent stem cell (iPSC)-derived mesenchymal stem cells (iMSCs) obtained using the neural crest cell (NCC) + PRIME protocol. iMSCs were obtained from canine embryonic fibroblast (CEF)-derived iPSCs (CEF-iPSCs), canine urine-derived cell (cUC)-derived iPSCs (cUC-iPSCs), and canine peripheral blood mononuclear cell (cPBMC)-derived iPSCs (cPBMC-iPSCs) using the NCC + PRIME protocol. The origin of PRIME-iMSCs is represented on the upper side of the pictures. (A) Von Kossa staining after <t>osteoblast</t> induction. Von Kossa staining identified hydroxyapatite crystals in the extracellular matrix of differentiated cells. Scale bar = 100 μm. qPCR for osteogenic markers, SPP1 and BGLAP , were shown below the staining. Black bars and white bars represent iMSCs before and after differentiation. Data are presented as relative quantification (RQ) for iMSCs before differentiation and as the mean ± standard deviation (n = 3). ∗p < 0.05. (B) Oil Red O staining after adipocyte differentiation. The cells formed Oil Red O-positive lipid vacuoles in the cytoplasm. Scale bar = 20 μm. qPCR for adipogenic marker, PLIN1 , was shown below the staining. Black bars and white bars represent iMSCs before and after differentiation. Data are presented as RQ for iMSCs before differentiation and as the mean ± standard deviation (n = 3). (C) Images of Alcian blue staining after chondrocyte induction. The iMSCs from all iPSC lines formed spheres and exhibited Alcian blue-positive proteoglycan production. Nuclei were stained red using nuclear fast red stain. Scale bar = 50 μm. qPCR for chondrogenic markers, ACAN and SOX9 , were shown below the picture. Black bars and white bars represent iMSCs before and after differentiation. Data are presented as RQ for iMSCs before differentiation and as the mean ± standard deviation (n = 3).
Osteoblast Differentiation Medium, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ogmtm osteoblast differentiation medium singlequotstm supplements  (Lonza)
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Lonza ogmtm osteoblast differentiation medium singlequotstm supplements
Tri-lineage differentiation ability of induced pluripotent stem cell (iPSC)-derived mesenchymal stem cells (iMSCs) obtained using the neural crest cell (NCC) + PRIME protocol. iMSCs were obtained from canine embryonic fibroblast (CEF)-derived iPSCs (CEF-iPSCs), canine urine-derived cell (cUC)-derived iPSCs (cUC-iPSCs), and canine peripheral blood mononuclear cell (cPBMC)-derived iPSCs (cPBMC-iPSCs) using the NCC + PRIME protocol. The origin of PRIME-iMSCs is represented on the upper side of the pictures. (A) Von Kossa staining after <t>osteoblast</t> induction. Von Kossa staining identified hydroxyapatite crystals in the extracellular matrix of differentiated cells. Scale bar = 100 μm. qPCR for osteogenic markers, SPP1 and BGLAP , were shown below the staining. Black bars and white bars represent iMSCs before and after differentiation. Data are presented as relative quantification (RQ) for iMSCs before differentiation and as the mean ± standard deviation (n = 3). ∗p < 0.05. (B) Oil Red O staining after adipocyte differentiation. The cells formed Oil Red O-positive lipid vacuoles in the cytoplasm. Scale bar = 20 μm. qPCR for adipogenic marker, PLIN1 , was shown below the staining. Black bars and white bars represent iMSCs before and after differentiation. Data are presented as RQ for iMSCs before differentiation and as the mean ± standard deviation (n = 3). (C) Images of Alcian blue staining after chondrocyte induction. The iMSCs from all iPSC lines formed spheres and exhibited Alcian blue-positive proteoglycan production. Nuclei were stained red using nuclear fast red stain. Scale bar = 50 μm. qPCR for chondrogenic markers, ACAN and SOX9 , were shown below the picture. Black bars and white bars represent iMSCs before and after differentiation. Data are presented as RQ for iMSCs before differentiation and as the mean ± standard deviation (n = 3).
Ogmtm Osteoblast Differentiation Medium Singlequotstm Supplements, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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osteoblastic differentiation  (Thermo Fisher)
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Thermo Fisher osteoblastic differentiation
Tri-lineage differentiation ability of induced pluripotent stem cell (iPSC)-derived mesenchymal stem cells (iMSCs) obtained using the neural crest cell (NCC) + PRIME protocol. iMSCs were obtained from canine embryonic fibroblast (CEF)-derived iPSCs (CEF-iPSCs), canine urine-derived cell (cUC)-derived iPSCs (cUC-iPSCs), and canine peripheral blood mononuclear cell (cPBMC)-derived iPSCs (cPBMC-iPSCs) using the NCC + PRIME protocol. The origin of PRIME-iMSCs is represented on the upper side of the pictures. (A) Von Kossa staining after <t>osteoblast</t> induction. Von Kossa staining identified hydroxyapatite crystals in the extracellular matrix of differentiated cells. Scale bar = 100 μm. qPCR for osteogenic markers, SPP1 and BGLAP , were shown below the staining. Black bars and white bars represent iMSCs before and after differentiation. Data are presented as relative quantification (RQ) for iMSCs before differentiation and as the mean ± standard deviation (n = 3). ∗p < 0.05. (B) Oil Red O staining after adipocyte differentiation. The cells formed Oil Red O-positive lipid vacuoles in the cytoplasm. Scale bar = 20 μm. qPCR for adipogenic marker, PLIN1 , was shown below the staining. Black bars and white bars represent iMSCs before and after differentiation. Data are presented as RQ for iMSCs before differentiation and as the mean ± standard deviation (n = 3). (C) Images of Alcian blue staining after chondrocyte induction. The iMSCs from all iPSC lines formed spheres and exhibited Alcian blue-positive proteoglycan production. Nuclei were stained red using nuclear fast red stain. Scale bar = 50 μm. qPCR for chondrogenic markers, ACAN and SOX9 , were shown below the picture. Black bars and white bars represent iMSCs before and after differentiation. Data are presented as RQ for iMSCs before differentiation and as the mean ± standard deviation (n = 3).
Osteoblastic Differentiation, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Tri-lineage differentiation ability of induced pluripotent stem cell (iPSC)-derived mesenchymal stem cells (iMSCs) obtained using the neural crest cell (NCC) + PRIME protocol. iMSCs were obtained from canine embryonic fibroblast (CEF)-derived iPSCs (CEF-iPSCs), canine urine-derived cell (cUC)-derived iPSCs (cUC-iPSCs), and canine peripheral blood mononuclear cell (cPBMC)-derived iPSCs (cPBMC-iPSCs) using the NCC + PRIME protocol. The origin of PRIME-iMSCs is represented on the upper side of the pictures. (A) Von Kossa staining after osteoblast induction. Von Kossa staining identified hydroxyapatite crystals in the extracellular matrix of differentiated cells. Scale bar = 100 μm. qPCR for osteogenic markers, SPP1 and BGLAP , were shown below the staining. Black bars and white bars represent iMSCs before and after differentiation. Data are presented as relative quantification (RQ) for iMSCs before differentiation and as the mean ± standard deviation (n = 3). ∗p < 0.05. (B) Oil Red O staining after adipocyte differentiation. The cells formed Oil Red O-positive lipid vacuoles in the cytoplasm. Scale bar = 20 μm. qPCR for adipogenic marker, PLIN1 , was shown below the staining. Black bars and white bars represent iMSCs before and after differentiation. Data are presented as RQ for iMSCs before differentiation and as the mean ± standard deviation (n = 3). (C) Images of Alcian blue staining after chondrocyte induction. The iMSCs from all iPSC lines formed spheres and exhibited Alcian blue-positive proteoglycan production. Nuclei were stained red using nuclear fast red stain. Scale bar = 50 μm. qPCR for chondrogenic markers, ACAN and SOX9 , were shown below the picture. Black bars and white bars represent iMSCs before and after differentiation. Data are presented as RQ for iMSCs before differentiation and as the mean ± standard deviation (n = 3).

Journal: Regenerative Therapy

Article Title: Generation of canine induced pluripotent stem cell-derived mesenchymal stem cells: Comparison of differentiation strategies and cell origins

doi: 10.1016/j.reth.2025.05.008

Figure Lengend Snippet: Tri-lineage differentiation ability of induced pluripotent stem cell (iPSC)-derived mesenchymal stem cells (iMSCs) obtained using the neural crest cell (NCC) + PRIME protocol. iMSCs were obtained from canine embryonic fibroblast (CEF)-derived iPSCs (CEF-iPSCs), canine urine-derived cell (cUC)-derived iPSCs (cUC-iPSCs), and canine peripheral blood mononuclear cell (cPBMC)-derived iPSCs (cPBMC-iPSCs) using the NCC + PRIME protocol. The origin of PRIME-iMSCs is represented on the upper side of the pictures. (A) Von Kossa staining after osteoblast induction. Von Kossa staining identified hydroxyapatite crystals in the extracellular matrix of differentiated cells. Scale bar = 100 μm. qPCR for osteogenic markers, SPP1 and BGLAP , were shown below the staining. Black bars and white bars represent iMSCs before and after differentiation. Data are presented as relative quantification (RQ) for iMSCs before differentiation and as the mean ± standard deviation (n = 3). ∗p < 0.05. (B) Oil Red O staining after adipocyte differentiation. The cells formed Oil Red O-positive lipid vacuoles in the cytoplasm. Scale bar = 20 μm. qPCR for adipogenic marker, PLIN1 , was shown below the staining. Black bars and white bars represent iMSCs before and after differentiation. Data are presented as RQ for iMSCs before differentiation and as the mean ± standard deviation (n = 3). (C) Images of Alcian blue staining after chondrocyte induction. The iMSCs from all iPSC lines formed spheres and exhibited Alcian blue-positive proteoglycan production. Nuclei were stained red using nuclear fast red stain. Scale bar = 50 μm. qPCR for chondrogenic markers, ACAN and SOX9 , were shown below the picture. Black bars and white bars represent iMSCs before and after differentiation. Data are presented as RQ for iMSCs before differentiation and as the mean ± standard deviation (n = 3).

Article Snippet: The cells were cultured in canine osteoblast differentiation medium (Cell Applications, San Diego, CA, USA) for 28 days at 37 °C and 5 % CO 2 .

Techniques: Derivative Assay, Staining, Quantitative Proteomics, Standard Deviation, Marker